Despite the significant progress in the fields of prosthetics and dental implantology, in many cases the replacement of missing teeth using conventional removable dentures is the only solution. The presence of a removable denture combined with insufficient oral hygiene may contribute to the development of stomatitis. The ability of bacteria and fungi to form a biofilm, which is closely related to prosthetic stomatopathy, plays a significant role in the pathogenesis of infections. The aim of the article is to present the current knowledge on the formation of biofilm and to review methods to prevent its deposition on removable dentures based on the related literature. Based on the narrative literature, various methods of preventing biofilm formation on the acrylic material as well as ways of its removal have been presented. Nowadays, researchers are optimistic regarding the modification of biomaterials in nanotechnology to reduce the adhesion of bacteria to the acrylic and the use of bacteriostatic and bactericidal nanocoatings to be applied to the acrylic material. The strategies for preventing biofilm formation described in the article represent the new methods of prevention that still require considerable research to enable their ultimate use in dentistry. At the moment, the most effective method of preventing biofilm formation is maintaining hygiene of the mouth and dentures.